Pengaruh Gliseraldehid pada Pemeriksaan Kolesterol Total, HDL, LDL, dan Trigliserida Serum

ANIK WIDIJANTI, RATULANGI BERNARD T., TINNY ENDANG HERNOWATI

Laboratorium Patologi Klinik RSU Dr. Saiful Anwar/FKUB, Malang.

Abstract

Background: Glyceraldehydes is an antiglycolitic agent, which has more benefit than other antiglycoli­tic agents. Glyceraldehydes does not interfere several parameter methods, which done with glucose, such urea, bilirubine, albumin, total protein, alkaline phosphatase, AST (Aspartate transaminae), ALT (alanine transaminase) and electrolyte. In diabetes mellitus (DM) patients require to check the level of total cholesterol, HDL (high density lipoprotein), LDL (low density lipoprotein) and triglyceride, to predict complication. In this study, we want to know whether glyceraldehydes interfere levels of tota­l cholesterol, HDL, LDL cholesterol and triglyceride.

Material and method: Patient’s whole blood was divided into 2 groups. One group was added with 10 mmol/L glyceraldehydes and one group was none. Then we took the serum and check level of total cholester­ol, HDL, LDL cholesterol and triglyceride. Student t-test was used to compare the result and the bias of these methods were measured.

Results: in group with no glyceraldehydes, the mean of total cholesterol was 227.58+39.24 mg/dl, HDL 47.6+8.17 mg/dl, LDL 149.78+37.42 mg/dl, and triglyceride 218.84+127 mg/dl. In group with glyceraldehyd­es the mean of total cholesterol was 215.68+37.72 mg/dl, HDL 46.64+8.28 mg/dl, LDL 142.6+36.05 mg/dl, and triglyceride 214.54+133.99 mg/dl. Statistically, the total cholesterol, LDL cholester­ol were differ significantly (p=0.000) but not in HDL cholesterol and triglyceride level (p 0.133 and 0.476 respectively). Nevertheless, the bias of total and LDL cholesterol still not exceed reference bias allowed.

Conclusion: Glyceraldehydes can used in whole blood and does not interfere the level of total cholesterol, HDL, LDL cholesterol and triglyceride.

Abstrak

Latar belakang: gliseraldehid merupakan antiglikolitik yang tidak mempengaruhi pemeriksaan ureum, bilirubin, albumin, protein total, fosfatase alkali, transaminase (AST, ALT), dan elektrolit. Pada diabete­s mellitus (DM), selain gula darah perlu juga diperiksa kolesterol total, LDL, HDL, dan trigliserida untuk memprediksi terjadinya komplikasi. Karena itu, perlu diteliti apakah penambahan gliseraldehid pada sampel darah tidak mempengaruhi hasil pemeriksaan kadar kolesterol total, HDL, LDL, dan trigliserida.

Metodologi: sampel darah utuh pasien dari poli penyakit dalam, dibagi menjadi 2. Kelompok I diberi antiglikolitik gliseraldehid dengan kadar 10 mmol/L dan  kelompok II tanpa gliseraldehid. Kedua serum diperiksa kadar kolesterol total, HDL, LDL, dan trigliserida. Hasil pemeriksaan kedua kelompok dibanding­kan dengan menggunakan t-test.

Hasil: pada kelompok I didapatkan kadar rerata kolesterol total 215,68+37,72 mg/dl; HDL 46,64+8,28 mg/dl; LDL 142,6+36,05 mg/dl; dan trigliserida 214,54+133,99 mg/dl. Sedangkan kelompok II didapat­kan kadar rerata (mean) kolesterol total 227,58+39,24 mg/dl; HDL 47,6+8,17 mg/dl; LDL 149,78+37,42 mg/dl; dan trigliserida 218,84+127 mg/dl. Hasilnya menunjukkan bahwa pemeriksaan HDL dan trigliserida tidak berbeda bermakna antara kedua kelompok (p=0,133 dan p=0,476), sedangkan kolesterol total dan LDL didapatkan perbedaan bermakna (p=0,000). Tetapi, perbedaan hasil tersebut tidak melebihi bias metode pemeriksaan yang diperbolehkan.

Kesimpulan: penambahan gliseraldehid pada sampel darah utuh tidak mempengaruhi pemeriksaan kadar kolesterol total, HDL, LDL, dan trigliserida.

Kata kunci: Gliseraldehid, antiglikolitik, kolesterol total, HDL, LDL, trigliserida

(Anik Widijanti dkk., Medika 2011, Tahun ke XXXVII, No. 10, p. 672–677)

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